Donnerstag, 02. Oktober 2014, 14:30 - 15:45 iCal

"The Monkey King and Pigsy ferrying the proteomic sutras into the third millennium"

Prof. Pier-Giorgio Righetti / Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano



Hörsaal II der Fakultät für Chemie
Währinger Straße 42, 1090 Wien


About fifty years of progress in “proteome” analysis, starting from primitive two-dimensional (2D) maps attempts in the early sixties of last century, will be here covered. The polar star in 2D mapping arose in 1975 with the classical paper by O’Farrell in J Biol. Chem. It became the compass for all proteome navigators. Perfection only came, though, with the introduction of immobilized pH gradients, which fixed the polypeptides spots in the 2D plane. Great impulse in proteome analysis came by introducing informatics tools and creating databases, among which Swiss Prot remains the site of excellence. Towards the end of the nineties, 2D chromatography, as epitomized by coupling strong cation exchangers with C18 resins, began to be a serious challenge to electrophoretic 2D mapping, although up to the present both techniques are still much in vogue and appear to give complementary results. Yet the migration of “proteomics” into the third millennium was only made possible by mass spectrometry (MS, the Monkey King!), which today represents the standard analytical tool in any lab dealing with proteomic analysis. Another major improvement has been the introduction of combinatorial peptide ligand libraries (CPLL, Pigsy!), which, when properly used, enhance the visibility of low-abundance species by 3 to 4 orders of magnitude. Coupling the Monkey King with Pigsy permits to explore at least 8 orders of magnitude in dynamic range on any proteome.


Fakultät für Chemie


Brigitte Schwarz
Fakultät für Chemie der Universität Wien