Dienstag, 19. Juni 2018, 15:00 - 16:30 iCal

Guest talk: Dr. Fabio Gallazzi

University of Missouri, Columbia

“Preparation of optimized biomolecules for multiple se-lected research projects”

Hörsaal 4 der Fakultät für Chemie
Währinger Straße 42, 1090 Wien

Lecture

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We optimized synthetic strategies and analytical / preparative purification conditions to prepare a variety of biomolecules. These were subsequently used in multiple research studies. A few examples will be described in detail as outlined below. First, short linear and longer cyclized peptides were prepared with a sequence which is expected to bind with Magnesium and subsequently trap CO2 as an example of application in envi-ronmental chemistry. Second, we designed, synthesized and characterized several chimera conjugates con-stituted by Peptide Nucleic Acids (PNA) antisense sequences covalently connected to cell penetrating pep-tides and functionalized either with fluorescent labeling or with bifunctional chelating agents able to bind radiometals. These conjugates were then used for imaging or therapeutic applications in cancer research. Finally we are currently optimizing native chemical ligation techniques for preparation of longer polypep-tides (65 to 130 amino acids) for a variety of potential applications in biochemistry and immunology. The use of an in-house optimized preparative purification system obtained by connecting a preparative HPLC system to a Mass spec ion trap has proved instrumental for the isolation of these products from a complex reaction mixture which otherwise might have been untreatable.

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Veranstalter

Fakultät für Chemie, Institut für Anorganische Chemie

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Kontakt

Lena Yadlapalli
Dekanat der Fakultät für Chemie
+43-1-4277-52012
lena.yadlapalli@univie.ac.at

Erstellt am Dienstag, 15. Mai 2018, 12:08
Letzte Änderung am Mittwoch, 16. Mai 2018, 10:00